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Dr. Des Raj Kashyap

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deskashyap@gmail.com

PROFESSIONAL EXPERIENCE

University of North Carolina at Chapel Hill, Chapel Hill, NC-27510 (Jan 2007-till date)

Montana State University, Bozeman, MT, Post Doctoral Fellow (September, 2004-Dec 2006). Working on identification of the determinants for arsenite [As (III)] oxidation in Agrobacterium tumefaciens soil isolate using transposon mutagenesis technique. We have been able to identify Tn5-B22 insertions in three unique mutants. Transposon insertions are located in modB, encodingthe permease component of a high affinity molybdate transport operon, and the mnhB gene which is part of a seven gene operon coding for Na+:H+ antiporter in Staphylococcus aureus. In a third mutant, the transposon inactivates an ntr homologue that is immediately upstream of the AsIII oxidaes structural gene, AroAB. We are also interested in demonstrating the chemoautotrophic growth by the wild type strain using AsIII as a primary source of energy.    

 

Panjab University, Chandigarh, India, Senior Research Associate  Worked on anaerobic digestion of solid waste at psychrophilic temperature. This job included isolation of bacterial strains growing at psychrophilic temperature and evaluation of there potential to produce methane at this temperature. Kinetic studies of the single stage and two stage anaerobic digestion processes. Based on the kinetic studies, fabrication of pilot scale digester for the process.

 

Panjab University, Chandigarh, India. Guest Faculty. Taught Microbiology to the Undergraduate classes (BE, Food Technology) in Department of chemical Engineering and Technology, Panjab University, Chandigarh, India, for Six Months.

 

Academic Training

Panjab University, Chandigarh, India.    PhD      2003       Production, purification, characterization and applications of an alkalophilic, thermotolerant pectinase from a mesophilic Bacillus sp. DT7”, Advisors:  Prof. R. Tewari and Dr. S. K. Soni 

Panjab University, Chandigarh, India.      MSc        1998       Salmonella typhi OMP’s induced alterations in murine macrophages”, Advisor: Dr. Praveen Rishi.

Panjab University, Chandigarh, India.      BsC        1995       Microbiology

 

Poster PRESENTED

R.Tewari, D. R. Kashyap. Commercial applications of alkalophilic and thermotolerant pectinase produced by a mesophilic Bacillus sp. CT16. Poster presentation in the section of applied microbiology in Canadian Society of Microbiologist-Annual General Meeting on June 10-13 (2001) at University of Waterloo, Canada.

T. R. McDermott, D. R. Kashyap, L. M. Botero and W. Franck. Identification and initial characterization of novel gene loci involved in regulation and function of arsenite oxidation in Agrobacterium tumefaciens. Jackson Hole, Wyoming.

Symposium/Seminar Attended 

      National Symposium on “Role of Microbes in the Management of Environmental Pollution”, held at Deptt. of Microbiology, P.U. Chandigarh on Nov. 13-14, 1998.

      Third Punjab Science Congress and Symposium on “100 Years of Post-Mendelian Genetics: Advent of Biotechnology”, held at Panjab University, Chandigarh on Dec., 10-12, 1999.

     “Scientific Interaction Program”, organized by Indian Science Congress Association at the Deptt. Of Biotechnology, Panjab University, Chandigarh on Feb., 26, 1999.

     National conference on “Electron Microscope and Allied Science”, held at Central Instrumentation Laboratories (CIL), Panjab University, Chandigarh from  9-11 Feb. 2001.

      91st National Scince Conference at Panjab University, Chandigarh from 3-7th January 2004.

Professional Memberships

Member All India Biotechlogy Association (AIBA).

 

PUBLICATIONS


PUBLISHED             


1.      Kashyap, D. R., Sood. S, Tiwari, R.P. and Rishi. P. (2000). Salmonella typhi OMP,s induced   immunomodulations in peritoneal macrophages. Indian J. Exp. Biol. 38: 358-362.


2.      Kashyap, D. R., Chandra, S., Kaul, A. and Tewari, R. (2000). Production, purification and characterization of pectinase from a Bacillus sp. DT7. World J. Micobiol. Biotechnol. 16: 277-282.


3.      Kashyap, D. R., Vohra, P.K., Chopra, S. and Tewari, R. (2001). Applications of pectinases in the commercial sector: A review. Biores. Technol. 77 (3): 215-227.


4.      Kashyap, D. R., Vohra, P.K., Soni, S.K and Tewari, R. (2001). Degumming of buel (Grewia optiva) bast fibres by pectinolytic enzyme from Bacillus sp. DT7. Biotechnol. Lett. (23): 1297-1301.


5.      Kashyap, D. R., Dadhich, K. S. and Sharma, S. K. (2003). Biomethanation under psychrophilic conditions: A Review. Biores. Technol. 87: 147-153.


6.      Kashyap, D. R., Soni, S. K. and Tewari, R. (2003). Enhanced production of alkaline and thermotolerant pectinase by Bacillus sp. DT7 in solid state fermentation. Biores. Technol. 80 (3): 251-254.


7.      Vohra, P.K., Sharma, R.K., Kashyap, D. R. and Tewari, R. (2001). Enhanced production of penicillin G acylase from a recombinant E. coli. Biotechnol. Lett. 27(3): 531-535.


8.      U. Ahuja, Vohra, P.K., Kashyap, D. R. and Tewari, R. (2001) Adsorption of heavy metals (Pb2+ and Cd2+) by free and immobilized biomass of Acinetobacter anitratus. Indian J.  Microbiology. 41: 229-233.


9.      U. Ahuja, Vohra, P. K. Kashyap, D. R. and Tewari, R. (2001) Removal of heavy metals (Pb2+ and Cd2+) from dilute solutions by free and immobilized Bacillus sp UA-12. Indian J. Environmental Sciences. 5(2): 131-137.



10   Kashyap, D. R., Botero, L. M., Franck, W. L., Hasset, D. J. and McDermott, T. R. (2004). Complex regulation of Arsenite Oxidation in Agrobacterium tumefaciens. J. Bacteriology. 188 (3): 1081-1088.


11.  Kashyap, D. R., Botero, L. M., Lehr, C., Hasset, D. J. and McDermott, T. R. (2004). A Na+:H+ Antiporter and Molybdate Transport are essential For Arsenite Oxidation in Agrobacterium tumefaciens. J. Bacteriology. 188 (4): 1577-1584


11.  1. Lehr, L. M., Kashyap, D. R, and McDermott, T. R. (2007). New Insights into Microbial Oxidation of Antimony and Arsenic. App. Env. Microbio. March 2007.


 


PERSONAL


BIRTH                                                            30th August, 1969


NATIONALITY                                              Indian


MARRITAL STATUS                                   Married