University of North Carolina
at Chapel Hill, Chapel Hill, NC-27510 (Jan 2007-till date)
Montana State University, Bozeman, MT, Post Doctoral Fellow (September, 2004-Dec 2006). Working on identification of the determinants for arsenite [As (III)] oxidation in Agrobacterium tumefaciens
soil isolate using transposon mutagenesis technique. We have been able to identify Tn5-B22 insertions in three unique mutants.
Transposon insertions are located in modB, encodingthe permease component of a high affinity molybdate transport
operon, and the mnhB gene which is part of a seven gene operon coding for Na+:H+ antiporter in
Staphylococcus aureus. In a third mutant, the transposon inactivates an ntr homologue that is immediately
upstream of the AsIII oxidaes structural gene, AroAB. We are also interested in demonstrating the chemoautotrophic
growth by the wild type strain using AsIII as a primary source of energy.
Chandigarh, India, Senior Research Associate Worked on anaerobic digestion of solid waste at psychrophilic temperature. This job
included isolation of bacterial strains growing at psychrophilic temperature and evaluation of there potential to produce
methane at this temperature. Kinetic studies of the single stage and two stage anaerobic digestion processes. Based on the
kinetic studies, fabrication of pilot scale digester for the process.
University, Chandigarh, India.
Guest Faculty. Taught Microbiology to the Undergraduate classes (BE, Food Technology) in Department
of chemical Engineering and Technology, Panjab University,
Chandigarh, India, for Six Months.
University, Chandigarh, India. PhD 2003 “Production,
purification, characterization and applications of an alkalophilic, thermotolerant pectinase from a mesophilic Bacillus
sp. DT7”, Advisors: Prof. R. Tewari and Dr. S. K. Soni
Chandigarh, India. MSc
1998 “Salmonella typhi
OMP’s induced alterations in murine macrophages”, Advisor: Dr. Praveen Rishi.
Chandigarh, India. BsC
R.Tewari, D. R. Kashyap. Commercial applications of alkalophilic and
thermotolerant pectinase produced by a mesophilic Bacillus sp. CT16. Poster presentation in the section of applied microbiology in Canadian Society of Microbiologist-Annual General Meeting
on June 10-13 (2001) at University of Waterloo, Canada.
T. R. McDermott, D. R. Kashyap, L. M. Botero and W. Franck. Identification
and initial characterization of novel gene loci involved in regulation and function of arsenite oxidation in Agrobacterium
tumefaciens. Jackson Hole, Wyoming.
· National Symposium on “Role of Microbes in the Management of Environmental Pollution”, held at Deptt. of Microbiology, P.U. Chandigarh on Nov. 13-14, 1998.
Punjab Science Congress and Symposium on “100 Years of Post-Mendelian Genetics: Advent of Biotechnology”,
held at Panjab University, Chandigarh
on Dec., 10-12, 1999.
· “Scientific Interaction Program”, organized by Indian Science Congress Association at the Deptt.
Of Biotechnology, Panjab University,
Chandigarh on Feb., 26, 1999.
· National conference on “Electron Microscope and Allied Science”, held at Central Instrumentation
Laboratories (CIL), Panjab University,
Chandigarh from 9-11 Feb. 2001.
· 91st National Scince Conference at Panjab University,
Chandigarh from 3-7th
Member All India Biotechlogy Association (AIBA).
D. R., Sood. S, Tiwari, R.P. and Rishi. P. (2000). Salmonella typhi OMP,s induced immunomodulations
in peritoneal macrophages. Indian J. Exp. Biol. 38: 358-362.
D. R., Chandra, S., Kaul, A. and Tewari, R. (2000). Production,
purification and characterization of pectinase from a Bacillus sp. DT7. World J. Micobiol. Biotechnol. 16: 277-282.
D. R., Vohra, P.K., Chopra, S. and Tewari, R. (2001).
Applications of pectinases in the commercial sector: A review. Biores. Technol. 77 (3): 215-227.
4. Kashyap, D. R., Vohra, P.K., Soni, S.K and Tewari, R. (2001). Degumming of buel (Grewia optiva) bast fibres by pectinolytic enzyme from Bacillus sp. DT7. Biotechnol. Lett. (23): 1297-1301.
D. R., Dadhich, K. S. and Sharma, S. K. (2003). Biomethanation under psychrophilic
conditions: A Review. Biores. Technol. 87: 147-153.
D. R., Soni, S. K. and Tewari, R. (2003). Enhanced production of alkaline and
thermotolerant pectinase by Bacillus sp. DT7 in solid state fermentation. Biores.
Technol. 80 (3): 251-254.
P.K., Sharma, R.K., Kashyap, D. R. and Tewari, R. (2001). Enhanced production
of penicillin G acylase from a recombinant E. coli. Biotechnol. Lett. 27(3): 531-535.
8. U. Ahuja, Vohra, P.K., Kashyap,
D. R. and Tewari, R. (2001) Adsorption of heavy metals (Pb2+ and Cd2+) by free and immobilized biomass
of Acinetobacter anitratus. Indian J. Microbiology. 41: 229-233.
U. Ahuja, Vohra, P. K. Kashyap, D. R. and Tewari, R. (2001) Removal of heavy metals (Pb2+ and Cd2+) from dilute
solutions by free and immobilized Bacillus sp UA-12. Indian J. Environmental Sciences. 5(2): 131-137.
10 Kashyap, D. R., Botero, L. M., Franck, W. L., Hasset, D. J. and McDermott, T.
R. (2004). Complex regulation of Arsenite Oxidation in Agrobacterium tumefaciens. J. Bacteriology. 188
11. Kashyap, D. R., Botero, L. M.,
Lehr, C., Hasset, D. J. and McDermott, T. R. (2004). A Na+:H+ Antiporter and Molybdate Transport are essential For Arsenite
Oxidation in Agrobacterium tumefaciens. J. Bacteriology. 188 (4): 1577-1584
11. 1. Lehr, L. M., Kashyap, D. R, and McDermott,
T. R. (2007). New Insights into Microbial Oxidation of Antimony and Arsenic. App. Env. Microbio. March 2007.
30th August, 1969